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Image Search Results
Journal: Plant Methods
Article Title: An improved method of constructing degradome library suitable for sequencing using Illumina platform
doi: 10.1186/s13007-019-0524-7
Figure Lengend Snippet: The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small RNA library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) EcoP15I digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
Article Snippet: TRIzol ® reagent (ThermoFisher, Cat. No. 15596-026) Dynabeads ® mRNA purification kit (ThermoFisher, Cat. No. 610-06) 3 M sodium acetate (NaOAc), pH = 5.5, RNase-free (ThermoFisher, Cat. No. AM9740) Primers from TruSeq ® small RNA sample prep kit-Set A (Illumina, Cat. No. RS-200-0012) T4 DNA ligase (ThermoFisher, Cat. No. 15224-017)
Techniques: Sequencing, Generated, Isolation, Ligation, Amplification, Purification
Journal: Plant Methods
Article Title: An improved method of constructing degradome library suitable for sequencing using Illumina platform
doi: 10.1186/s13007-019-0524-7
Figure Lengend Snippet:
Article Snippet: TRIzol ® reagent (ThermoFisher, Cat. No. 15596-026) Dynabeads ® mRNA purification kit (ThermoFisher, Cat. No. 610-06) 3 M sodium acetate (NaOAc), pH = 5.5, RNase-free (ThermoFisher, Cat. No. AM9740) Primers from TruSeq ® small RNA sample prep kit-Set A (Illumina, Cat. No. RS-200-0012) T4 DNA ligase (ThermoFisher, Cat. No. 15224-017)
Techniques:
Journal: Plant Methods
Article Title: An improved method of constructing degradome library suitable for sequencing using Illumina platform
doi: 10.1186/s13007-019-0524-7
Figure Lengend Snippet:
Article Snippet: TRIzol ® reagent (ThermoFisher, Cat. No. 15596-026) Dynabeads ® mRNA purification kit (ThermoFisher, Cat. No. 610-06) 3 M sodium acetate (NaOAc), pH = 5.5, RNase-free (ThermoFisher, Cat. No. AM9740) Primers from TruSeq ® small RNA sample prep kit-Set A (Illumina, Cat. No. RS-200-0012) T4 DNA ligase (ThermoFisher, Cat. No. 15224-017)
Techniques:
Journal: Plant Methods
Article Title: An improved method of constructing degradome library suitable for sequencing using Illumina platform
doi: 10.1186/s13007-019-0524-7
Figure Lengend Snippet:
Article Snippet: TRIzol ® reagent (ThermoFisher, Cat. No. 15596-026) Dynabeads ® mRNA purification kit (ThermoFisher, Cat. No. 610-06) 3 M sodium acetate (NaOAc), pH = 5.5, RNase-free (ThermoFisher, Cat. No. AM9740) Primers from TruSeq ® small RNA sample prep kit-Set A (Illumina, Cat. No. RS-200-0012) T4 DNA ligase (ThermoFisher, Cat. No. 15224-017)
Techniques: