platinum e cells cell biolabs cat Search Results


96
Selleck Chemicals cisplatin selleck chemicals cat
Cisplatin Selleck Chemicals Cat, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs t4 rna ligase
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
T4 Rna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Cell Biolabs Inc plat e ecotropic packaging cells
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
Plat E Ecotropic Packaging Cells, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc platinum e cells
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
Platinum E Cells, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc platinum e retroviral packaging cell line
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
Platinum E Retroviral Packaging Cell Line, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc platinum e plat e retroviral packaging cells
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
Platinum E Plat E Retroviral Packaging Cells, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc platinum e plat e retroviral packaging cell line
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
Platinum E Plat E Retroviral Packaging Cell Line, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc hek293t platinum e plat e retroviral packaging line
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
Hek293t Platinum E Plat E Retroviral Packaging Line, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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Cell Biolabs Inc ecotropic
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
Ecotropic, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/platinum+e+cells+cell+biolabs+cat/ecotropic/pmc13199673-42-5-7
Average 86 stars, based on 1 article reviews
ecotropic - by Bioz Stars, 2026-10
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k-562  (ATCC)
99
ATCC k-562
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
K 562, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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293t  (ATCC)
99
ATCC 293t
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
SignaGen transfection reagent polyjet
The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small <t>RNA</t> library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) <t>EcoP15I</t> digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform
Transfection Reagent Polyjet, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small RNA library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) EcoP15I digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform

Journal: Plant Methods

Article Title: An improved method of constructing degradome library suitable for sequencing using Illumina platform

doi: 10.1186/s13007-019-0524-7

Figure Lengend Snippet: The scheme for constructing improved degradome library. For sequencing purposes, the degradome library generated by this method can be treated as small RNA library and the resulting reads are ~ 27 nt long. The procedure includes: (1) poly(A) RNA isolation; (2) 5′RNA adapter ligation to uncapped poly(A) RNA with 5′ monophosphate; (3) reverse transcription to generate 1st strand cDNA using an oligo(dT)-tailed adapter (RT-primer); (4) second strand synthesis (1st PCR amplification); (5) EcoP15I digestion to generate ~ 27 nt long reads; (6) ligation of EcoP15I digestion products with a 3′ds-DNA adapter; (7) purification of ligation products on a PAGE gel; (8) degradome library enrichment (2nd PCR amplification); (9) purification of the final product on a PAGE gel; (10) library pooling and sequencing using Illumina HiSeq platform

Article Snippet: TRIzol ® reagent (ThermoFisher, Cat. No. 15596-026) Dynabeads ® mRNA purification kit (ThermoFisher, Cat. No. 610-06) 3 M sodium acetate (NaOAc), pH = 5.5, RNase-free (ThermoFisher, Cat. No. AM9740) Primers from TruSeq ® small RNA sample prep kit-Set A (Illumina, Cat. No. RS-200-0012) T4 DNA ligase (ThermoFisher, Cat. No. 15224-017) T4 RNA ligase (NEB, Cat. No. M0204S) EcoP15I (NEB, Cat. No. R0646S) Platinum ® Taq DNA polymerase high fidelity (ThermoFisher, Cat. No. 11304011) 20 bp DNA ladder (Takara, Cat. No. 3420A) 50 bp DNA ladder (Takara, Cat. No. 3421A) DL 1000 DNA ladder (Takara, Cat. No. 3591A) SuperScript™ II reverse transcriptase (ThermoFisher, Cat. No. 18064) RNaseOUT™ recombinant ribonuclease inhibitor (ThermoFisher, Cat. No. 10777-019) 40% Acrylamide/Bis19:1 40% (w/v) solution (ThermoFisher, Cat. No. 9022) SYBR™ gold nucleic acid gel stain (ThermoFisher, Cat. No. S11494) Ethidium bromide solution (Promega, Cat. No. H5041) DEPC-treated water (ThermoFisher, Cat. No. AM9906) Glycogen (ThermoFisher, Cat. No. 10814-010) MinElute ® PCR purification kit (QIAGEN, Cat. No. 28004) Corning_Costar_Spin-X_centrifuge tube filters (Sigma, Cat. No. CLS8162-24EA)

Techniques: Sequencing, Generated, Isolation, Ligation, Amplification, Purification

Journal: Plant Methods

Article Title: An improved method of constructing degradome library suitable for sequencing using Illumina platform

doi: 10.1186/s13007-019-0524-7

Figure Lengend Snippet:

Article Snippet: TRIzol ® reagent (ThermoFisher, Cat. No. 15596-026) Dynabeads ® mRNA purification kit (ThermoFisher, Cat. No. 610-06) 3 M sodium acetate (NaOAc), pH = 5.5, RNase-free (ThermoFisher, Cat. No. AM9740) Primers from TruSeq ® small RNA sample prep kit-Set A (Illumina, Cat. No. RS-200-0012) T4 DNA ligase (ThermoFisher, Cat. No. 15224-017) T4 RNA ligase (NEB, Cat. No. M0204S) EcoP15I (NEB, Cat. No. R0646S) Platinum ® Taq DNA polymerase high fidelity (ThermoFisher, Cat. No. 11304011) 20 bp DNA ladder (Takara, Cat. No. 3420A) 50 bp DNA ladder (Takara, Cat. No. 3421A) DL 1000 DNA ladder (Takara, Cat. No. 3591A) SuperScript™ II reverse transcriptase (ThermoFisher, Cat. No. 18064) RNaseOUT™ recombinant ribonuclease inhibitor (ThermoFisher, Cat. No. 10777-019) 40% Acrylamide/Bis19:1 40% (w/v) solution (ThermoFisher, Cat. No. 9022) SYBR™ gold nucleic acid gel stain (ThermoFisher, Cat. No. S11494) Ethidium bromide solution (Promega, Cat. No. H5041) DEPC-treated water (ThermoFisher, Cat. No. AM9906) Glycogen (ThermoFisher, Cat. No. 10814-010) MinElute ® PCR purification kit (QIAGEN, Cat. No. 28004) Corning_Costar_Spin-X_centrifuge tube filters (Sigma, Cat. No. CLS8162-24EA)

Techniques:

Journal: Plant Methods

Article Title: An improved method of constructing degradome library suitable for sequencing using Illumina platform

doi: 10.1186/s13007-019-0524-7

Figure Lengend Snippet:

Article Snippet: TRIzol ® reagent (ThermoFisher, Cat. No. 15596-026) Dynabeads ® mRNA purification kit (ThermoFisher, Cat. No. 610-06) 3 M sodium acetate (NaOAc), pH = 5.5, RNase-free (ThermoFisher, Cat. No. AM9740) Primers from TruSeq ® small RNA sample prep kit-Set A (Illumina, Cat. No. RS-200-0012) T4 DNA ligase (ThermoFisher, Cat. No. 15224-017) T4 RNA ligase (NEB, Cat. No. M0204S) EcoP15I (NEB, Cat. No. R0646S) Platinum ® Taq DNA polymerase high fidelity (ThermoFisher, Cat. No. 11304011) 20 bp DNA ladder (Takara, Cat. No. 3420A) 50 bp DNA ladder (Takara, Cat. No. 3421A) DL 1000 DNA ladder (Takara, Cat. No. 3591A) SuperScript™ II reverse transcriptase (ThermoFisher, Cat. No. 18064) RNaseOUT™ recombinant ribonuclease inhibitor (ThermoFisher, Cat. No. 10777-019) 40% Acrylamide/Bis19:1 40% (w/v) solution (ThermoFisher, Cat. No. 9022) SYBR™ gold nucleic acid gel stain (ThermoFisher, Cat. No. S11494) Ethidium bromide solution (Promega, Cat. No. H5041) DEPC-treated water (ThermoFisher, Cat. No. AM9906) Glycogen (ThermoFisher, Cat. No. 10814-010) MinElute ® PCR purification kit (QIAGEN, Cat. No. 28004) Corning_Costar_Spin-X_centrifuge tube filters (Sigma, Cat. No. CLS8162-24EA)

Techniques:

Journal: Plant Methods

Article Title: An improved method of constructing degradome library suitable for sequencing using Illumina platform

doi: 10.1186/s13007-019-0524-7

Figure Lengend Snippet:

Article Snippet: TRIzol ® reagent (ThermoFisher, Cat. No. 15596-026) Dynabeads ® mRNA purification kit (ThermoFisher, Cat. No. 610-06) 3 M sodium acetate (NaOAc), pH = 5.5, RNase-free (ThermoFisher, Cat. No. AM9740) Primers from TruSeq ® small RNA sample prep kit-Set A (Illumina, Cat. No. RS-200-0012) T4 DNA ligase (ThermoFisher, Cat. No. 15224-017) T4 RNA ligase (NEB, Cat. No. M0204S) EcoP15I (NEB, Cat. No. R0646S) Platinum ® Taq DNA polymerase high fidelity (ThermoFisher, Cat. No. 11304011) 20 bp DNA ladder (Takara, Cat. No. 3420A) 50 bp DNA ladder (Takara, Cat. No. 3421A) DL 1000 DNA ladder (Takara, Cat. No. 3591A) SuperScript™ II reverse transcriptase (ThermoFisher, Cat. No. 18064) RNaseOUT™ recombinant ribonuclease inhibitor (ThermoFisher, Cat. No. 10777-019) 40% Acrylamide/Bis19:1 40% (w/v) solution (ThermoFisher, Cat. No. 9022) SYBR™ gold nucleic acid gel stain (ThermoFisher, Cat. No. S11494) Ethidium bromide solution (Promega, Cat. No. H5041) DEPC-treated water (ThermoFisher, Cat. No. AM9906) Glycogen (ThermoFisher, Cat. No. 10814-010) MinElute ® PCR purification kit (QIAGEN, Cat. No. 28004) Corning_Costar_Spin-X_centrifuge tube filters (Sigma, Cat. No. CLS8162-24EA)

Techniques: